Using recombined SSB antigen expressed in Pichia pastoris to detect anti-SSB antibody by dot immunogold filtration assay
- VernacularTitle:利用酵母重组SSB抗原建立快速斑点免疫金渗滤法检测抗SSB抗体
- Author:
Xiuyun XU
;
Xiangyue YANG
;
Xiaopeng LAN
- Publication Type:Journal Article
- Keywords:
autoimmune diseases;
anti-SSB antibody;
dot immunogold filtration assay
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new,rapid,simple and reliable assay for detecting autoantibody SSB.Methods A new dot immunogold filtration assay(DIGFA) was developed,in which the recombinant SSB protein expressed in Pichia pastoris was bound to nitrocellulose(NC) membrane and colloidal gold-labeled staphylococus protein A(SPA) was used as an indicator.Results The sensitivity and specificity of DIGFA were 100% and 98.75%,respectively.The agreement between DIGFA and ENA dot assay was 99.01%.Conclusion DIGFA for detecting autoantibody SSB is a good,rapid,simple and accurate assay for clinical diagnosis.
