FRNK inhibited hepatic stellate cells in vitro
- VernacularTitle:FRNK抑制体外肝星状细胞增殖
- Author:
Jiangang SHEN
;
Xiaolan ZHANG
;
Xiaoxia HUO
;
Qinghai JIAO
;
Hongfang WANG
- Publication Type:Journal Article
- Keywords:
hepatic stellate cell;
proliferation;
FAK related non-kinase;
focal adhesion kinase;
extracellular signal-regulated kinase
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of FRNK on the proliferation of Hepatic Stellate Cells in vitro.Methods FRNK plasmid mediated by cationic liposome was transfected into HSCs.The proliferation of HSCs was evaluated by modified MTT assay.The level of FRNK,FAK,p-FAK(Tyr397),ERK1 and p-ERK in HSCs were assayed by Western blot and RT-PCR.Results Compared with nFRNK plasmid group,FRNK inhibited the proliferation of HSCs and the inhibition rates at 12,24 and 48 h were 20.07%,26.16% and 29.77%(P
