Effect of PARP inhibitor 5-AIQ on PARP/NF-?B complex and NF-?B activity in murine colon carcinoma CT26 cells
- VernacularTitle:PARP抑制剂5-AIQ对小鼠结肠癌CT26细胞PARP/NF-?B复合物和NF-?B活性的影响
- Author:
Li CAI
;
Yalan WANG
;
Xiao LIN
- Publication Type:Journal Article
- Keywords:
PARP;
5-AIQ;
NF-?B;
colon carcinoma
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of PARP inhibitor 5-AIQ on PARP/NF-?B P65 complex and the NF-?B activity in murine colon carcinoma CT26 cells.Methods The murine colon carcinoma CT26 cells were treated with 5-AIQ in vitro.The expressions of PARP and NF-?B P65 were analyzed by Western Blot.The PARP/NF-?B P65 complex was analyzed by co-immunoprecipitation.The NF-?B binding activity was detected by electrophoretic mobility shift assay(EMSA).Results Compared with 5-AIQ-untreated group,the expressions of PARP and NF-?B P65 were markedly decreased in 5-AIQ-treated colon carcinoma CT26 cell groups(P
