Environmental protection and economical procedures of tissue processing
- VernacularTitle:组织标本处理的经济环保流程
- Author:
Jun YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(46):-
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:The traditional tissue processing procedures,which includes a variety of toxic chemical reagents,both for himself and for operating environment there is a lot of damage.OBJECTIVE:To improve the traditional tissue processing procedures,to establish economic processing of tissue sample disposal,and to eliminate damages to the handlers and environment bringing by related reagents.DESIGN,TIME AND SETTING:The tissue sample processing study was performed at the Department of Pathology,Third Xiangya Hospital,Central South University from February 1998 to February 2008.MATERIALS:All specimens were routine surgical specimens of the Third Xiangya Hospital,Central South University.Acetic acid,alcohol,turpentine and paraffin(at 56-58 ℃) were obtained from Chemical Plant of Hunan Normal University,China.The automatic dehydration machine ZT-12P was purchased from Hubei Xiaogan Yaguang Medical Technology Co.,Ltd.,China.METHODS:Formaldehyde and xylene were replaced by non-toxic chemical reagents.Processing was as follows,0.7 volume fraction of ethanol and glacial acetic acid for 2 hours,0.8 volume fraction of for 1 hour,0.9 volume fraction of ethanol for 1 hour,0.95 volume fraction of ethanol Ⅰ for 2 hours,0.95 volume fraction of ethanol Ⅱ for 2 hours,dehydrated alcohol Ⅰ for 1 hour,dehydrated alcohol Ⅱ for 1 hour,turpentine oil Ⅰ for 1 hour,turpentine oil Ⅱ for 1 hour,paraffin Ⅰ for 1 hour,paraffin Ⅱ for 1 hour,and paraffin Ⅲ for 1 hour.After collecting,samples were put in automatic dehydration machine.Suitable for all kinds of human samples.MAIN OUTCOME MEASURES:To compare the tissue specimens processed by new program with the traditional processing.RESULTS:Specimens treated by improved environmental protection procedures can be sliced up smoothly,and dyed uniformity,which have marked contrast of red and blue.CONCLUSION:Improved environmental protection procedures can avoid handlers exposing to toxic chemical reagents,can entirely replace traditional procedures.
