Dose-effects of insulin-like growth factor-1 on proliferation of rat nucleus fibrosus cells in vitro
- VernacularTitle:胰岛素样生长因子Ⅰ对大鼠纤维环细胞体外增殖活性影响的量效关系
- Author:
Guangji KONG
;
Xun MA
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(46):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Recently,in vitro nucleus gelatinosus and annulus fibrosus cell culture technology,especially cartilage tissue engineering development and primary success in autogenous intervertebral disc cell transplantation to repair nucleus pulposus defect,brings a hope for regeneration and reparation of degenerated intervertebral disc.OBJECTIVE:To explore the dose-dependent and time-dependent effects of insulin-like growth factor-Ⅰ(IGF-Ⅰ) on proliferation of anulus fibrosus cells in vitro.DESIGN,TIME AND SETTING:Single-sample observation was performed at the Laboratory of Parasite,Shanxi Medical University between September 2006 and January 2007.MATERIALS:Thirty 1-month-old Wistar rats,irrespective of gender,were selected.METHODS:Anulus fibrosus cells were isolated and cultured in vitro.In dose-dependent test,IGF-Ⅰ(0.1,1.0,10,and 100 ?g/L),prepared by volume fraction 0.01 or 0.1 calf serum HAMF-12,were added.Cells not with IGF-Ⅰ served as control.Cells were cultured for 72 hours.In time-dependent test,optimal dose of IGF-Ⅰ containing volume fraction 0.1 calf serum and F-12 solution was added,and cells not with IGF-Ⅰ served as control.The cells were cultured for 1,3,5,and 7 days.MAIN OUTCOME MEASURES:Cell biological features were detected using HE staining and toluidine blue immunohistochemical staining;Dose-dependent and time-dependent effects were examined by MTT assay.RESULTS:HE stained cells were fusiform-shaped with pseudopodia,round or oval nucleus.The cytoplasm was blue after stained with the toluidine blue.The immunohistochemical test revealed that there existed positive expression of typeⅠcollagen in the cells.In the presence of 10% calf serum,IGF-Ⅰ significantly improved cell proliferative activity in a dose-dependent manner within effective dose range.CONCLUSION:IGF-Ⅰ can stimulate cell proliferation of rat anulus fibrosus cells in vitro in a dose-dependent and time-dependent manner.
