Study on human umbilical cord blood cells transplantation for treatment of experimental autoimmune encephalomyelitis
- VernacularTitle:人脐血干细胞移植治疗实验性自身免疫性脑脊髓炎的研究
- Author:
Fenghua PAN
;
Haixia DING
;
Xinsheng DING
- Publication Type:Journal Article
- Keywords:
human umbilical cord blood cells;
transplantation;
experimental autoimmune encephalomyelitis
- From:
Journal of Clinical Neurology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of human umbilical cord blood cells(HUCBCs) transplantation to treat experimental autoimmune encephalomyelitis(EAE) rats and the status of transplanted HUCBCs in the brain and spinal cord tissue of EAE rats.Methods The mononuclear cells abstracted from cord blood of infants were cultured in vitro and marked with 5-bromodeoxyuridine(Brdu) for 48 h.EAE rat models were made and the HUCBCs(3?106) were transplanted into the tail vein(transplanted group) 14 d later.The score of neurological function dificit and the number of the demyelinated foci in brain and spinal cord were undertaken at different time point after transplantation.The statue of survival,differentiation and migration of HUCBCs in vivo were determined by immunohistochemical technique,and compared with control group.Results The scores of neurological function dificit at 21 d,28 d post transplantation in transplanted group were much lower than those in the control group(all P
