Depletion of human serum albumins by ethanol precipitation
- VernacularTitle:乙醇沉淀法去除人血清清蛋白的实验研究
- Author:
Jun PAN
;
Xiaoyang LUO
;
Haiquan CHEN
- Publication Type:Journal Article
- Keywords:
Albumin;
Precipitation;
Proteomics
- From:
Journal of Medical Postgraduates
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effectiveness of ethanol precipitation in depleting the human serum albumin. Methods: Serum samples from 8 healthy controls and 8 lung adenocarcinoma patients were treated with 100% ethanol to deplete the albumin.The results of the albumin depletion were analyzed by 1-D gel electrophoresis and albumin quantitation. Results: After ethanol treatment,1-D gel images of the serum samples showed a near depletion of albumins a presence of low-abundance proteins.There was no difference in the 1-D gel images between the normal and lung adenocarcioma serum samples.The average concentrations of albumin in the ethanol-treated serum samples were(5.88 ? 0.35) g/L and(5.50 ? 0.53) g/L respectively in the control and lung adenocarcinoma groups.Conclusion: Ethanol precipitation can deplete most of the human serum albumins and thereby assist us in further analysis of human serum proteomics.
