Matrine induces apoptosis of glioma cell C6 and the possible mechanism
- VernacularTitle:苦参碱诱导胶质瘤C6细胞凋亡及其可能的基因机制
- Author:
Shujie ZHANG
;
Xinhua WANG
;
Binglin CHENG
- Publication Type:Journal Article
- Keywords:
matrine;
glioma cell;
apoptosis;
differential expression of genes;
caspase-3
- From:
Chinese Journal of Cancer Biotherapy
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the apoptosis inducing effect of matrine on C6 glioma cells and the related mechanisms.Methods:MTT assay was used to examine the inhibition of C6 glioma cell line by matrine at various concentrations and the IC50 was calculated.Inverted microscope and TEM(transmission electron microscope) were employed to observe the morphological alterations of C6 glioma cells after exposure to matrine;FCM(Flow cytometry) was used to detect the apoptosis rate of C6 glioma cells;and real-time PCR was used to examine the differential expression of related genes.ICC and Western blotting was used to detect the expression of caspase-3.Results:MTT showed that the cell inhibition effect of matrine increased with its concentration(0.1-1.0 mg/ml)(P
