Detection of Double Mutation of Basal Core Promoter(BCP) of HBV by TaqMan MGB
- VernacularTitle:TaqMan MGB探针检测HBV DNA C区双突变方法的建立
- Author:
Jianwei SHEN
;
Lihong TIAN
;
Yuanzhi WU
;
Donge CHENG
;
Xu WANG
;
Xiuqin SUN
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
TaqMan MGB probe;
HBV;
DNA;
Mutation
- From:
Chinese Journal of Nosocomiology
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a simple,sensitive method for detecting the double mutation of the basal core promoter(BCP) of HBV.METHODS FAM fluorescence-labeled TaqMan MGB and primers driving from the region containing the double mutation of BCP were designed for the real time PCR,then the standard positive control,standard negative control and HBV DNA were amplified and detected by the real time PCR.The results of detecting the double mutation of BCP were validated by the direct-sequencing analysis of PCR products.RESULTS The double mutation of BCP of HBV could be detected by the real time PCR.The sensitivity of the method was 3?100 copy templates and as few as 1% of mutant among wild-type virus sequence were detected.CONCLUSIONS The method can be used to detect the double mutation of BCP of serum HBV DNA.
