Detection of a New Type of Aminoglycoside-modifying Enzymes in a Pan-resistant Pseudomonas putida
- VernacularTitle:从全耐药恶臭假单胞菌检出一种氨基糖苷类修饰酶aac(6′)-Ⅰb基因的新变型基因
- Author:
Desong MING
;
Jianliang ZHUANG
;
Zhijun SU
;
Zhishan ZHANG
;
Zunjin XIE
- Publication Type:Journal Article
- Keywords:
Pan-resistant;
Pseudomonas putida;
Aminoglycoside-modifying enzymes
- From:
Chinese Journal of Nosocomiology
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study resistant mechanisms in a pan-resistant Pseudomonas putida strain.METHODS To detect the susceptibility of antimicrobial agents by MIC,6 aminoglycoside-modifying enzymes(AMEs) genes of 1 strain of P.putida were measured by PCR,and verified by DNA sequencing and similarity searches of DNA sequencing data banks using BLAST.RESULTS In the strain,there were 4 positive kinds of AMEs resistant genes(aac(6′)-Ⅰb,aac(3)-Ⅱ,ant(3″)-Ⅰand ant(2″)-Ⅰ).Sequence analysis revealed that the 4 PCR products were AMEs,the aac(6′)-Ⅰb was a new type(GenBank EU 137667).CONCLUSIONS The mechanisms of resistance to aminoglycosides of the pan-resistant P.putida are mainly related to 4 kinds of AMEs,the aac(6′)-Ⅰb is a new type.
