Influence of glypican-3 gene on proliferation,adhesion and invasion of hepatoma carcinoma cancer cell line SK-Hep-1
- VernacularTitle:磷脂酰肌醇蛋白聚糖-3基因对肝癌细胞SK-Hep-1黏附和侵袭的影响
- Author:
Bing WANG
;
Shan LIN
;
Lie WANG
- Publication Type:Journal Article
- Keywords:
glypican-3(GPC3);
SK-Hep-1 hepatoma carcinoma cell;
Invasion;
proliferation
- From:
Chinese Journal of Cancer Biotherapy
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of GPC3 gene on the proliferation,adhesion and invasion of hepatoma cell line SK-Hep-1.Methods:SK-Hep-1 cells were transfected with pEGFP-N2-GPC3 using Lipofectamine2000.RT-PCR was used to examine the GPC3mRNA expression in GPC3-SK-Hep-1 cells.MTT assay was used to examine the proliferation and calculate the adhesion rate of SK-Hep-1 cells.Transwell system was used to assess the migration and invasion of the cells.Results:SK-Hep-1 cells were successfully transfected with pEGFP-N2-GPC palsmid.GPC3 mRNA was detected in SK-Hep-1 cells.Transfection with CPC3 significantly suppressed the growth of SK-Hep-1 cells(P
