Interaction Between Anthracene Quinone Type Anti-tumor Antibiotics and DNA
- VernacularTitle:蒽醌类抗肿瘤抗生素与DNA相互作用探究
- Author:
Dan SHEN
;
Yanxia LU
;
Xiuluan ZHANG
;
Huiping SUN
;
Fang ZHANG
- Publication Type:Journal Article
- Keywords:
Anthracene quinone type anti-tumor antibiotics;
DNA;
Interaction;
Research
- From:
Chinese Journal of Nosocomiology
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the interaction of anthracene quinone type anti-tumor antibiotics and DNA as well as the experiment method.METHODS Spectrophotometry was developed including absorption spectrum,fluorescence spectrum,electrochemical method and so on.RESULTS The union of inlay and insertion observed in ultraviolet and visible spectrophotometry usually caused hypochromic effect and red shift.Under the certain concentrations of bovin serum albumin(BSA),the endogene fluorescence intensity of BSA orderly reduced with the increase in concentration of doxorubicin(adriamycin) hydrochloride(?em 344 and the peak shape were invariable);?ex and ?em at the biggest wave length of doxorubicin were 478 and 596 nm.The fluorescence intensity was maximal of the excitation and emission spectrum when pH was 3.0.CONCLUSIONS The interaction of doxorubicin and DNA is the strongest according to the experiment and is the most widely used at present in clinics.
