Differentiation of rats bone marrow mesynchymal stem cells into cardiomyogenic cells with pacemaking function
- VernacularTitle:大鼠骨髓间充质干细胞体外诱导分化为具有起搏功能的心肌细胞
- Author:
Xin WEI
;
Lianfeng CHEN
;
Bojiang LIU
;
Quan FANG
- Publication Type:Journal Article
- Keywords:
mesynchymal stem cell;
lysate of the sinoatrial cell;
hyperpolarization-activated cyclic nucleotide-gated channel(HCN)
- From:
Basic & Clinical Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find the way of inducing the bone marrow mesynchymal stem cells(MSCs)into cardiac cells with pacemaking function in vitro.Methods Dissociate the rat MSCs and induce them with 5AZA,bFGF+EGF,HGF,SCF and lysate of the sinoatrial cells respectively.The morphological changes were observed,and the expressing of protein cTnT,connexin 43 and HCN2/4 were analyse by immunohistologic and flowcytometry techniques.The pacmaking current If were evaluted by patch clamp techniques.Results All the methods can induce the bone marrow MSCs to differentiate into cardiac cells,which expressing cardiac cell specific protein and HCN2.Cells induced by 5AZA,bFGF+EGF and SAN CMs show higher rate of HCN2 expressing(22.9%,22.3%,11%).The cells of these groups have the pacmaking current If.Conclusion Lysate of the sinoatrial cells are ideal methods of inducing the bone marrow MSCs to differentiate into cardiac cells with pacemaking function in vitro.HCN is a promising marker protein to select pacmaking cells out of the differentiated cells.
