The secreted expression of PSP94 in E. coli.
- Author:
Changsheng TIAN
;
Ruijuan GAO
;
Xuejian ZHAO
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(8):781-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: PSP94 has been shown promise as a potential prostate cancer marker and it was reported that PSP94 can inhibit the growth of prostate cancer cell in vitro and in vivo. This study aimed to construct recombinant human PSP94 expression vector. METHODS:The PSP94 cDNA was obtained from normal prostate tissue, and recombinant plasmid pUC19-PSP94 was constructed. The target gene was identified and sequenced. Then the PSP94 gene was inserted to the secretory expression vector. RESULTS:The gene sequence of PSP94 was identified. The recombinant vector was constructed. The secreted PSP94 was isolated and identified by Western blot. CONCLUSION:The recombinated PSP94 could expressed PSP94 successfully.
