Effect of Bak on mitochondrial morphplogy in apoptosis
- VernacularTitle:Bak在细胞凋亡期间对线粒体形态学的影响
- Author:
Leping FENG
;
Wei QIAO
;
Brooks CRAIG
;
Dong ZENG
- Publication Type:Journal Article
- Keywords:
apoptosis;
cytochrome C;
mitochondria;
Bax gene,Bak gene
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the function of Bak in mitochondrial signaling pathway and interaction between Bax and Bak during apoptosis.Methods Bax/Bak double knock out(Bax-/- and Bak-/-)MEF cells from mouse embryo fibroblasts(MEF) and Hela cells were divided into four groups according the cell different genotypes(wild type,Bax-/-,Bak-/- and double knock out) and treated with different chemical reagents after co-transfection with Bax,Bak,Mito-Red and empty pEGFP vector.Apoptosis,mitochondrial morphology and cytochrome C release were detected with confocal microscope,immunofluoresence and Western blotting techniques.Results There were correlations between the percentage of Hela cell apoptosis and mitochondrial fission(%) as well as cytochrome C(%)(P
