Identification and characterizaton of mitochondrial DNA and cytochrome b patial gene in Martes zibellina L.
- VernacularTitle:貂组织线粒体DNA及细胞色素b鉴定及特征
- Author:
Lihua ZHANG
;
Mingcheng LI
;
Bingmei WANG
- Publication Type:Journal Article
- Keywords:
Martes zibellina L.;
DNA,mitochondrial;
cytochrome b
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the characterization of mitochondrial DNA and cytochrome b(Cytb) gene from Martes zibellina L..Methods The mtDNA were extracted from fresh heart and liver of Martes zibellina L.by alkaline lysis method respectively,and Cyt b gene segment was amplified by PCR technique and sequenced.Results The complete 16800 bp mtDNA was separated from both samples,and the Cytb gene with 310 bp segments was amplified by PCR and the sequence indicated that the homologous similarity was 99% with sable Martes zibellina L.(GenBank:AB026109.1) and it was 45% with other animal similarity.Conclusion There is complete mtDNA in tissues of Martes zibellina L.and Cytb partial gene from martes zibellina L.may be used as the genetic marker for identification of different species.
