Effect of insulin-like factor-Ⅰ on proliferation activity of human periodontal ligament fibroblasts
- VernacularTitle:胰岛素样生长因子Ⅰ对人牙周膜成纤维细胞增殖的影响
- Author:
Yungang LUO
;
Xiaoqiu LIU
;
Xiaomin ZHU
;
Xiaodong YAN
- Publication Type:Journal Article
- Keywords:
insulin-like faotor-Ⅰ;
periodontal ligament fibroblost;
transfection
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of insulin-like factor-I(IGF-Ⅰ)on the proliferation activity of human periodontal ligamental fibroblasts(PDLF).Methods The PDLF transfected with IGF-Ⅰ and PDLF untransfected with IGF-Ⅰ prepared in our lab were digested by 0.25% trypsin and prepared into cell suspension.The two sorts of cells were counted after being vaccinated,then the growth curves of the two cells were mapped and the growth speeds were compared.The PDLF transfected with IGF-Ⅰ and PDLF untransfected with IGF-Ⅰ were digested by 0.25% trypsin and then cultivated.The proliferation activities of the PDLF transfected with IGF-Ⅰ and PDLF untransfected with IGF-Ⅰ were observed using MTT methods.The activity of alkaline phosphatase(ALP) was determined by using ALP kit.Results The growth curves of the two cells showed that the growth speed of the PDLF transfected with IGF-Ⅰ was significantly faster than that of the PDLF untransfected with IGF-Ⅰ(P
