Establishment of HEK293 cell lines stably expressing m-nAChR
- VernacularTitle:稳定表达肌肉型乙酰胆碱受体的细胞株建立和鉴定
- Author:
Bin YANG
;
Yachun ZHOU
;
Shitong LI
- Publication Type:Journal Article
- Keywords:
Acetylcholine receptor;
HEK293 cell;
Gene transfection;
Immunohistochemistry
- From:
Journal of Medical Postgraduates
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish human embryonic kidney(HEK) 293 cell lines that that can stably express the muscle nicotinic acetylcholine receptor(AChR).Methods: The pcDNA3.1 plasmid was constructed and transfected into HEK293 cells with lipofectin,the stable transfectants screened by G418 and the protein expression identified and analyzed by immunohistochemistry.Results: After G418 screening,14 of the transfected cell lines highly expreesed ?-nAchR and 4 showed an obvious expression of ?-nAChR,as demonstrated by the immunohistochemical analysis.Conclusion: HEK293 cell lines stably expressing m-nAChR were constructed successfully.
