Biological phenotype and cell cycle distribution characteristics of nasal and/or nasopharyngeal epithelia in TgN(p53mt-LMP1)/HT mice
- VernacularTitle:TgN(p53mt-LMP1)/HT小鼠鼻腔和鼻咽黏膜上皮组织生物学表型特征及细胞周期特点分析
- Author:
Yingchun HE
;
Daofa TIAN
;
Hongping LIU
;
Fangguo LU
;
Xinzheng WU
- Publication Type:Journal Article
- Keywords:
Biological phenotype;
Cell cycle;
Precancerous lesion;
Transgenic mouse
- From:
Journal of Medical Postgraduates
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the biological phenotype and cell cycle distribution characteristics of nasal and/or nasopharyngeal epithelia of TgN(p53mt-LMP1)/HT mice.Methods: The biological phenotype changes(mainly the incidence of precancerous lesion) in nasal and nasopharyngeal epithelial tissues of the generation G4 of TgN(p53mt-LMP1)/HT mice aged 5,11,15 and 18 months were determined by H-E staining,and the cell cycle characteristics of nasal and nasopharyngeal epithelia detected by flow cytometry(FCM).Results: The incidences of precancerous lesion in the nasal and nasopharyngeal epithelia were 0,50%,60% and 75% respectively in the 5,11,15 and 18 mos groups of the positively expressed transgenic mice,and 0 in the four age groups of the negatively expressed ones.Compared with the negatively expressed transgenic mice,the number of nasal or nasopharyngeal epithelial cells was markedly decreased in the G0/G1 phase,but obviously increased in the S and G2/M phases,with the proliferation index(PI) significantly enhanced(P
