Effect of recombinant human granulocyte colony-stimulating factor on new microvessels in rat perihematoma after intracerebral hemorrhage
- VernacularTitle:人重组粒细胞集落刺激因子对大鼠脑出血周围区新生血管的影响
- Author:
Xiaoying HE
;
Hua TAN
;
Ping YUAN
;
Xiaogang LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(38):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Recombinant human granulocyte colony-stimulating factor (rhG-CSF) can mobilize endothelial progenitor cells and enhance new vessels at cerebral ischemia region. OBJECTIVE: To investigate the effects of rhG-CSF on the new microvascular expressions in rat perihematoma after intracerebral hemorrhage. DESIGN, TIME AND SETTING: A randomized control animal experiment was performed at the Central Laboratory of Luzhou Medical College from March to November 2006. MATERIALS: A total of 72 healthy male Sprague Dawley rats and rhG-CSF were used for this study. METHODS: Seventy-two rats were equally and randomly assigned into the sham operation group, the hemorrhage group, the treatment group. According to rat brain stereotaxic atlas, models of intracerebral hemorrhage were made by infusing autoblood from rat tails. Rats in the sham operation group were infused with saline instead of autoblood. Rats in the treatment group were administered rhG-CSF (60 ?g/kg) by intraperitoneal injection at 1 hour after operation. Rats in the sham operation and hemorrhage groups were left intact. MAIN OUTCOME MEASURES: The microvascular expressions of CD34+ in perihematoma were detected at 6, 12, 24, 48 and 72 hours, 7 days; Four rats in each time point. Microvascular production was measured by changes in CD34. The more the CD34 antigens, the more the new vessels were. RESULTS: In the hemorrhage group, the microvascular expressions of CD34+ were significantly higher compared to the sham operation group (P 0.05). Significant differences were measured at 6, 12, 24 and 48 hours (P
