Screen and Identification of The Protein-protein Interactors in The Host Cell With The SARS Coronavirus Nucleocapsid Protein
- VernacularTitle:宿主细胞内SARS-CoV N蛋白相互作用蛋白的筛选与鉴定
- Author:
Weishan CHANG
;
Jing ZHAI
;
Wengang SONG
;
Yongqing LIU
- Publication Type:Journal Article
- Keywords:
SARS_CoV nucleocapsid protein, host cell protein-protein interactor, CXCL16
- From:
Progress in Biochemistry and Biophysics
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
15 SARS-CoV N Protein Interacting Protein (NPIP) were selected from host cells using Yeast Two-hybrid system (Y2H). These are Angiogenin, acyglycerol kinase, cytochrome oxydase subunit I, CXC chemokine ligand 16, epidermal growth factor receptor pathway substrate 15, glutathione S-transferase kappa 1,integrin beta 1, jun oncogene, NIMA (never in mitosis gene a)-related kinase 10, protein tyrosine kinase 2 beta,homo sapiens SH3KBP1 binding protein 1 and ubiquitin specific peptidase 53. With the aid of immunological co-precipitation (CO-IP), it was confirmed that chemokine CXCL16 was the interactor with SARS-CoV N protein in host cells.
