Development of a Rapid System for Bacteria Identification in 24 Hours
- VernacularTitle:建立24h快速病原菌检测系统的研究
- Author:
Xianbin LI
;
Rui ZHAO
;
Caizhi HUANG
;
Yuan LAI
;
Yulian JIANG
;
Sai LI
;
Xunliang JIANG
- Publication Type:Journal Article
- Keywords:
Rapid system for bacteria identification;
Drug susceptibility test;
Revolving;
TTC
- From:
Chinese Journal of Nosocomiology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop a rapid system for bacteria identification and susceptibility test in 24 hours,and provide bacteriological evidence for the control of nosocomial infection and the timely diagnosis and treatment.METHODS The simple constant temperature box was replaced by revolving constant temperature box;2,3,5-triphenyltetrazolium chloride(TTC) and succinate were added to the culture bottle and the culture medium of drug susceptibility;the concentration of the reactive substrate in the bacterial biochemical tube and the number of the inoculated bacteria were increased at the same time.RESULTS The time of positive blood culture in the revolving constant temperature box was significantly shorter than that in the simple one(?2=74.92,P