Double protecting functions of rat Sertoli cells to co-encapsulated hepatocytes in vitro
- VernacularTitle:大鼠睾丸支持细胞对体外共微囊化肝细胞的双重保护作用
- Author:
Hailong LIN
;
Yongping CHEN
;
Minghua ZHENG
;
Yuee HE
;
Yu HUANG
;
Lanman XU
- Publication Type:Journal Article
- Keywords:
hepatocyte;
Sertoli cell;
co-encapsulation;
immune privilege
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the nourishment and immune privilege effects of Sertoli cells on co-encapsulated hepatocytes. Methods The hepatocytes and Sertoli cells were encapsulated or co-encapsulated in various ratio of 100∶1、50∶1、20∶1、10∶1, and co-cultured for 21 days in vitro. The secretion of albumin and urea was analyzed, and the morphology of encapsulated cells was observed by microscopy, then to determine the best mixed ratio of hepatocytes to Sertoli cells. Splenocyte proliferation response was assessed to evaluate Sertoli cell’s immune privilege function to hepatocytes by CCK-8.Results Sertoli cells could elevate hepatocyte’s secretion of albumin and urea when they were co-encapsulated with each in appropriate ratio (P
