Recombinant adenovirus vector-delivered report gene expression in rats kidney through different ways
- VernacularTitle:重组腺病毒载体介导报告基因经多种途径转染至大鼠肾脏
- Author:
Lin LI
;
Fang WU
;
Hong LI
- Publication Type:Journal Article
- Keywords:
recombinant adenovirus;
kidney;
gene;
rat
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate expression of recombinant adenovirus(rAV) vector-mediated report gene in kidney, and to seek for the best passway of rAV vector-mediated gene transfer in vivo.Methods Thirty Sprague-Dawley(SD) rats were divided into 5 groups randomly. rAV-GFP(1013viral particales?L-1) was injected through different ways. 2, 7 and 14 d after injection, GFP expression in left kidney, right kidney, liver and lung was evaluated by fluorescence microscope and computer software. Results GFP gene expressed in kidey and reached peak 7 d after injection expression. Conclusion rAV vector-mediated report gene can be transducted in kidney. The rAV-mediated transgene expression in kidney is a potential strategy in the treatment of renal diseases.
