Interleukin-1? up-regulates the expression of VEGF in A549 cell line
- VernacularTitle:白介素-1?上调肺腺癌细胞系A549 VEGF的表达
- Author:
Lanyan ZHU
;
Ping CHEN
;
Yunsheng JIANG
- Publication Type:Journal Article
- Keywords:
lung cancer;
interleukin-1?;
vascular endothelial growth factor
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation effect of interleukin-1? (IL-1?) on the expression of VEGF in A549 cells. Methods A549 cells were incubated with IL-1? in the presence or absence of Celecoxib. After incubation, cells were harvested to detect VEGF mRNA by reverse transcription-polymerase chain reaction (RT-PCR), and the supernatant was collected to determine the concentration of VEGF protein by enzyme-linked immunosorbent assay (ELISA). Results After treatment with IL-1?, the expression of VEGF mRNA and protein in A549 cells were significantly higher than that in the control group(P
