Expression of galectin-10 in CD4~+ CD25~+ CD127~(low/-) regulatory T cells
- VernacularTitle:半乳凝素-10在CD4~+ CD25~+ CD127~(low/-)调节性T细胞中的表达与意义
- Author:
Jian WANG
;
Xiangliang YUAN
;
Jian XUE
;
Meixing LI
;
Lisong SHEN
- Publication Type:Journal Article
- Keywords:
galectin-10;
CD4+CD25+CD127low/-regulatory T cells;
Foxp3;
cell sorting
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of galectin-10 in CD4+CD25+CD127low/-regulatory T cells(Treg) and the correlation between expressions of galectin-10 and Foxp3.Methods CD4+CD25+CD127low/-Treg cells and CD4+CD25-CD127+ T cells were isolated from PBMC of healthy volunteers and lymph nodes of gastric cancer patients by flow cytometry.Following RNA micro-extraction RT-PCR analysis was used to detect mRNA expression of galectin-10 and Foxp3.Results The mRNA expression of galectin-10 and Foxp3 were dominant in Treg cells,whereas nearly no expression in CD4+CD25-CD127+ T cells.The results from PBMC of healthy volunteers and lymph nodes of gastric cancer patients were consistent between.Conclusion The expression of galectin-10 was obviously high in Treg cells,so it may become a novel marker for phenotypic characterization of Treg cells.
