Application of silica nanoparticles C-dots in fluorescent labeling for cells
- VernacularTitle:纳米荧光染料C-dots标记细胞的应用
- Author:
Tao ZHOU
;
Weiguo FU
;
Yuqi WANG
- Publication Type:Journal Article
- Keywords:
near-infrared fluorescence(NIRF);
C-dots;
cells labeling
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application of near infrared fluorescent dye C-dots in cell labeling.Methods B16 melanoma cells were cultured with C-dots of various diameters.The distribution of C-dots in cells was observed by laser confocal fluorescent microscope.The proliferation of both C-dots-labeled and unlabeled cells were measured by MTT colorimetry.The viability of labeled cells was checked by trypan blue staining.Results C-dots with diameter of 5 or 10 nanometers can be used to label cells.The near infrared fluorescence of C-dots was taken into the cells and observed on the cellular membrane.No difference of cellular viability was found between the labeled and unlabeled cells.Conclusion C-dots can be used as an intracellular marker for the research on trace and proliferation of cells.
