Analysis of Toxoplasma gondii Specific Antigens by Two Dimensional Electrophoresis and Western Blotting
- VernacularTitle:应用双向电泳及蛋白质印迹分析弓形虫特异性抗原
- Author:
Linjie LI
;
Huaien BAO
;
Wei PAN
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
Tachyzoite;
SD rat;
Antigen;
Two-dimensional electrophoresis;
Western blotting
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The total protein of Toxoplasma gondii RH strain tachyzoites was separated by two-dimensional electrophoresis (2-DE), and Western blotting was performed to find out distinct antigens. 209 spots were detected through Coomassie brilliant blue-stained small gels (7?8 cm, pH 3-10). Western blotting showed 17 specific antigen spots with sera of the experimental group and two nonspecific spots with the control sera.
