Dynamic Observation of Attachment and Invasion of Toxoplasma gondii Tachyzoites to Intestinal Mucosa in BALB/c Mice by Chromogenic In Situ Hybridization Targeting SAG2 mRNA
- VernacularTitle:RNA原位杂交法动态观察弓形虫速殖子对BALB/c小鼠小肠黏膜的黏附及侵入
- Author:
Xiaoming MA
;
Xiaoli MENG
;
Guorong YIN
;
Hongli LIU
;
Jinyan SHEN
- Publication Type:Journal Article
- Keywords:
RNA in situ hybridization;
Toxoplasma gondii;
Tachyzoite;
Intestinal mucosa;
Epithelial cell
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe dynamically the location and time of attachment and invasion of Toxoplasma gondii tachyzoites to murine intestinal mucosa by chromogenic in situ hybridization targeting SAG2 mRNA. Methods Thirty 7- to 8-week-old BALB/c mice were randomly divided into experiment group(24 mice)and control group(6 mice). Each animal in the experiment group was given 2?104 tachyzoites of RH stain in 0.2 ml PBS by intragastric administration and that in the control group was given 0.2 ml PBS. Four mice in the experiment group and one in the control group were sacrificed at 15 min,30 min,1 h,2 h,4 h and 8 h after infection,respectively,and paraffin sections of duodenum,jejunum and ileum were prepared to perform the in situ hybridization with Dig-labeled oligonucleotide probe complementary to SAG2 mRNA of T. gondii. Results Tachyzoites were found on the striated border of small intestine epithelial cells (absorptive cells,goblet cells and endocrine cells),in or between two absorptive cells or in the lamina propria. At 15 min-2 h after infection,there was significant difference in the number of attachment on jejunum and ileum (P
