Study of adipose-derived stromal cells transfected glial cell-derived neurotrophic factor by lentivirus in cerebral transplantation
- VernacularTitle:慢病毒转染胶质细胞源性神经营养因子脂肪干细胞脑移植的研究
- Author:
Bing SUN
;
Shixin GU
;
Ke WANG
- Publication Type:Journal Article
- Keywords:
lentivirus;
adipose-derived stromal cells;
transplantation
- From:
Journal of Clinical Neurology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the applied value of adipose-derived stromal cells(ADSCs) transfected glial cell-derived neurotrophic factor(GDNF)by lentivirus in cerebral transplantation.Methods Primarily isolated and cultured, the rat ADSCs were transfected GDNF by high-titer lentivirus. Then, configuration of cells and expression of neuron-specific enolase (NSE) were observed. By stereotaxic frame, transfected ADSCs were intrastriatally transplanted.1month and 2month after transplantation, survival and migration of marked cells were detected. GDNF expression, verified by Western blot, was compared with control group.Results ADSCs, transfected by lentivirus, can differentiate into neuron-like cells and express NSE in cytolymph. 1 month after transplantation, the majority of cells survived in transplanting area. 2 month later, part of transplanted cells still survived and migrated far from the transplanting area. In addition, the level of GDNF protein in the brain tissue was significantly higher compared with control group (all P
