Expression and Significance of Cyclin A1 mRNA in the Skin of Wild-Type Mice
- VernacularTitle:野生型小鼠皮肤cyclinA1 mRNA的表达及其意义
- Author:
Xia ZHU
;
Hongquan WEI
;
Yuanhong LI
;
Yan WU
;
Chen ZHAO
;
Xinghua GAO
;
Hongduo CHEN
- Publication Type:Journal Article
- Keywords:
CyclinA1 mRNA;
Skin;
Sebaceous gland;
Epidermis;
In situ hybridization;
Mice;
wild-type
- From:
Acta Laboratorium Animalis Scientia Sinica
2010;18(1):21-23,彩5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and significance of cyclin A1 in the skin of wild-type mice at RNA level.Methods Thirty 6-12-week old wild-type Kunming mice(15 male and 15 female)were included in this study.In situ hybridization was used to detect the expression of cyclin A1 mRNA in the skin of head and neck of the mice.The skin treated without probe was regarded as negative control and the testis of male mice was taken as positive contr01.Results The expression of cyclin A1 mRNA was found in sebaceous glands of 25 mice and in epidermis of 12 mice.Strong positive staining of sebaceous glands was seen in 50% and positive staining in 33.3% of sections,whereas strong positive and positive staining of epidermis was seen in 13.3% and 20% of sections.respectively.The positive rate of sebaceous glands was 83.3%,much higher than that of epidermis(33.3%).Conclusions There is a quite high expression of eyelin A1 mRNA in the skin sebaceous gland and epidermis of head and neck of wild-type mice.Especially a strong expression is in the sebaceous glands.It indicates that cyclin A1 mRNA may play a certain role in the physiological function of sebaceous glands and epidermis of the skin.
