Genotyping of Hepatitis C Virus and Analysis of The Molecular Evolution Based on Core Region Sequence
- VernacularTitle:丙型肝炎病毒基因分型及core蛋白区的分子演化分析
- Author:
Xianghui WAN
;
Zhaofang ZENG
;
Ximei YANG
- Publication Type:Journal Article
- Keywords:
hepatitis C virus,genotype,molecular evolution,core protein
- From:
Progress in Biochemistry and Biophysics
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
At present, there are many methods for genotype of hepatitis C virus , but not a gold standard. In order to establish the rationale for genotypic determination of optimal region sequence, fifteen complete genome sequences of hepatitis C which had been given the annotation about every region and derived from different country were downloaded from GenBank. Phylogenetic trees on 5' UTR, core, E1, E2 and NS5B region were established. The results demonstrated that genotyping group was not all correct on 5' UTR region while genotyping groups were wholly correct on core, E1, E2 and NS5B region. Comparing phylogenetic distances on core, E1, E2 and NS5B region with that on complete genome sequence demonstrated that the NS5B area was the best genotyping region instead of the complete genome sequence. In addition, analysis of the molecular evolution on each core region could supply some clues for creating novel genotyping method based on PCR-RFLP.
