Drug Resistance and Genotype of Extended Spectrum ?-Lactamases and Plasmid AmpC Enzyme-producing Klebsiella pneumoniae
- VernacularTitle:产ESBLs与质粒AmpC酶肺炎克雷伯菌的耐药性及基因型研究
- Author:
Meijie JIANG
;
Li FENG
;
Shuping ZHAO
;
Qiaoguang HAO
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
Extended spectrum ?-lactamases;
Plasmid;
AmpC enzymes
- From:
Chinese Journal of Nosocomiology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the drug-resistance,the existing forms,genotype,and transfer ways of extended spectrum ?-lactamases(ESBLs) and plasmid AmpC enzyme in Klebsiella pneumoniae.METHODS The drug sensitivity of K.pneumoniae to 17 antibiotics was done by slip-diffusion and microdilution methods.The genotype of two enzymes was assessed by PCR and sequencing.The transfer ways of K.pneumoniae drug-resistant gene were identified by transconjugants-test.RESULTS The ESBLs were mainly produced in 55 cefoxitin resistant K.pneumonia strains.The major genotypes of ESBLs and plasmid AmpC nzyme were CTX-M,MIR and DHA.These genes could be transferred from clinical isolates to recipient bacteria.CONCLUSIONS ESBLs as well as AmpC enzymes are the most important resistance mechanism in K.pneumoniae.The resistance could be transferred through the bacterial conjugation.
