Observation of Histomorphology and Biomechanics on Tendons Preserved by Vitrification
- VernacularTitle:玻璃化保存法对肌腱形态学及生物力学影响
- Author:
Wenliang WANG
;
Yingjie LIU
;
Hualiang ZHANG
;
Guang CHEN
- Publication Type:Journal Article
- Keywords:
tendon;
morphology;
vitodynamics;
vitrifiation;
cryopreservation
- From:
Chinese Medical Equipment Journal
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of vitrification on the biomechanics of tendon tissue in rabbit.Methods Two frozen methods were adopted.The first group was treated with Cryoprotective Agent,which was composed of 18.64%DMSO(V/V),13.37% Acetamide(V/V),9.17% 1,2 Propylene glyco(V/V),0.10mmol/LTrehalose and 10% Calf serum.The tendon tissue with three steps of preliminary treatment,preserved in Cryoprotective Agen was conserved in liquid nitrogen(-196℃)for 14 days;The second group treated with 15% DMSO and 10% Calf serum served as control group.Transmission electron microscope was used to observe the Histiooytic shape of tendon.Each group was performed with tendon tensile test,which could detect maximum load,the maximum shifting quantity and Young's Elastic Modulus.Results There was no significant damage in the tissue's micromechanism of vitrified tendon.But in cryopreservation group,the tissue's micromechanism was apparently damaged.There was no significant difference between test group and control group in maximum load(P=0.256).The same was the maximum shifting quantity(P=0.065).There was significant difference between test group and control group in Young's Elastic Modulus(P=0.006).Conclusion The damage of vitrification to tendon is less than that of profound hypothermia preservion,especially to tendon's corpuscular shape,but there is no significant difference between test group and control group in tendon's biomechanics.
