Recombinant adenovirus-mediated survivin gene transfection of dendritic cells induces immune responses against renal cell carcinoma
- VernacularTitle:重组腺病毒介导survivin基因转染树突状细胞对肾细胞癌的免疫效应
- Author:
Haibiao LAI
;
Song WU
;
Xiwen ZHONG
;
Liang ZHONG
;
Zhifeng HUANG
;
Ye ZENG
- Publication Type:Journal Article
- Keywords:
survivin gene;
dendritic cell;
renal cell carcinoma;
adenovirus;
immuno therapy;
gene therapy
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anti-tumor immune responses against renal cell carcinoma induced by dendritic cells(DCs)transfected with survivin gene mediated by recombinant adenovirus.Methods:The DCs derived from human peripheral blood were infected with recombined adenovirus vector carrying the survivin gene.The expression of survivin protein in the infected DCs was examined by Western blotting assay;the surface expression of CD83,MHCⅡ,CD80 and CD86 by flow cytometry(FCM),Interleukin-12(IL-12)in the supernatants of DCs and IFN-? released by the cytotoxic T lymphocytes(CTLs)by ELISA,the ability of DCs in proliferating allo-lymphocytes by mixed lymphocyte reaction(MLR),and specific killing activity of CTLs by MTT assay.Results:The DCs presented mature DCs phenotype after transfection with Ad-svv.The expression of survivin protein in transfected DCs was confirmed by Western blotting analysis.The IL-12 level in the supernatant of DCs transfected with Ad-svv was significantly higher than that transfected with empty vector(Ad-CMV,P
