Pyrrolidine dithiocarbamate,a inhibitor of NF-?B,enhances chemosensitivity of ovarian cancer cells to cisplatin
- VernacularTitle:NF-?B抑制剂二硫代氨基甲酸吡咯烷提高卵巢癌细胞顺铂化疗的敏感性
- Author:
Guohong LIU
;
Bo WANG
- Publication Type:Journal Article
- Keywords:
pyrrolidine dithiocarbamate(PDTC);
cisplatin;
ovarian cancer;
nuclear factor-kappa B;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the promoting effect of pyrrolidine dithiocarbamate(PDTC),an inhibitor of NF-?B,on the sensitivity of human ovarian cancer cell line SKOV-3 to chemoagent cisplatin and the possible mechanisms.Methods:SKOV-3 cells were treated with various concentrations of cisplatin and PDTC combination for different periods.Then the growth inhibition of SKOV-3 cells were observed by MTT assay,expression of I-?B? and P65 protein by Western blotting assay,and apoptosis of cells by flow cytometry.Results:Separate application of both cisplatin(0.1-10 ?g/ml)and PDTC(10-40 ?mol/L L)significantly inhibited the growth of SKOV-3 cells and caused cell apoptosis(P
