Inhibitory effect of recombinant mutant human tumor necrosis factor combined with cisplatin on transplanted Lewis lung carcinoma in mice
- VernacularTitle:rmhTNF-?协同顺铂对小鼠Lewis肺癌移植瘤的抑制
- Author:
Suju WEI
;
Nan ZHANG
- Publication Type:Journal Article
- Keywords:
recombinant mutant human tumor necrosis factor;
cisplatin;
Lewis lung carcinoma;
apoptosisChin J Cancer Biother,2008,15(4): 361-364
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of recombinant mutant human tumor necrosis factor(rmhTNF-?)combined with cisplatin(DDP)against transplanted Lewis lung carcinoma(LLC)in mice,and to explore the related mechanism.Methods:LLC cells were subcutaneously inoculated into C57BL/6 mice at the right axilla and the mice were randomly divided into 4 groups:sodium chloride(control group),DDP group,rmhTNF-? group,rmhTNF-? plus DDP group.On the 7th day after inoculation the diameter of the tumor reached 0.6 cm;the corresponding agents were intratumorally injected for 3 d.All mice were sacrificed 1 d later and the tumors were obtained,weighed,and the tumor inhibitory rate was calculated.Flow cytometric analysis was used to examine cells apoptosis and cell cycle.Expression of survivin mRNA was examined by RT-PCR.Results:(1)The tumor inhibitory rates of the DDP plus rmhTNF-? group(36.61%)was significantly higher than those of the DDP group(17.12%)and rmhTNF-? group(15.83%,P
