Chondrogenesis of rabbit mesenchymal stem cells by overexpressing Sox9 gene
- VernacularTitle:Sox9基因转染对骨髓间充质干细胞成软骨分化的影响
- Author:
Ziquan YANG
;
Junren HE
;
Gang LI
;
Shuhua YANG
;
Xiaochun WEI
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Transfection;
Sox9;
Chondrogenesis
- From:
Chinese Journal of Rheumatology
2008;12(7):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Sox9 gene overexpressian on chondrogenesis of esenchymal stem ceils (MSCs) in vitro. Methods Rabbit MSCs were obtained and purified by gradient centrifuge and adhesion culture in vitro. MSCs were transfected by recombinant Sox9 plasmid. RT-PCR and Western blot analysis were used for transfection confirmation. Chondrogenic molecules such as type I1 collagen,aggrecan and Sox9 were detected by immunohistology, RT-PCR and western blot. Cell proliferation was tested by MTT assay. Results MSCs were successfully transfected with Sox9 gene and verified by RT-PCR and Western blot analysis. The overexpression of Sox9 had no effect on the proliferation of MSCs. Transfeeted cells expressed more chondrogenic markers than the control groups. Conclusion Sox9 gene overexpression can enhance chondrogenic differentiation of MSCs.
