NCX and SERCA2a expression in myocardial tissues in rats with pressure overload-induced left ventricular hypertrophy
- VernacularTitle:压力负荷性左室肥厚大鼠心肌钠钙交换体和肌浆网钙泵的表达
- Author:
Dan SU
;
Jianhua CHENG
;
Yalei DAI
;
Dazheng WU
;
Hailian SHI
- Publication Type:Journal Article
- Keywords:
left ventricular hypertrophy;
heart function;
NCX;
SERCA2a;
gene expression
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the abnormity of heart function in rats with pressure overload-induced left ventricular hypertrophy and the changes of NCX,SERCA2a expression in myocardial tissues. Methods Cardiac hypertrophy was induced by clipping the abdominal aorta in rats. The cardiac hypertrophy was evaluated by Left ventricular weight index(LVWI,left ventricular weight/body weight). NCX, SERCA2a mRNA and protein expressions in left ventricular tissues were determined by half-quantitative RT-PCR and Western blot normalized to abundance of GAPDH mRNA and protein,respectively. Results LVSP and LVEDP were obviously enhanced(P
