Irradiated combined with antisense VEGF therapy for esophageal cancer xenografts
- VernacularTitle:反义VEGF寡核苷酸联合放射线抑制食管癌细胞移植瘤生长
- Author:
Wei FENG
;
Shuchai ZHU
;
Yuxiang WANG
- Publication Type:Journal Article
- Keywords:
esophageal cancer;
VEGF;
xenografts;
irradiation
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of antisense VEGF on radiation sensitivity of esophageal cancer cells in vivo.Methods Sixteen male BLBA/C/nu nude mice were randomly divided into 4 groups and prepared cells were injected subcutaneously on paw pat of mice(2?106/100 ?L/mice): The size of the subcutaneous tumors was measured before and after irradiation and transfection.The effect of treatment was evaluated by speed of tumor growth. The expression level of VEGF was examined in different groups by RT-PCR,western blotting respectively.Results The absolute growth delay (AGD)for antisene group, irradiation group and antisene plus irradiation group was 3.0?2.6, 10.1?5.4 and 27.4?3.1 days,and nominal growth delay (NGD) was 24.4?3.1 days. The enhancement factor of antisense VEGF for radiotherapy was 2.41. A decreased expression level of endogenous VEGF mRNA and protein were found in antisene group by RT-PCR and western blotting analysis.Conclusion Antisense VEGF and radiotherapy may enhance radiotherapic effect for esophageal cancer xenografts.
