Detection of PTEN gene promoter hypermethylation in lung cancer patients
- VernacularTitle:肺癌患者PTEN基因启动子高甲基化的检测
- Author:
Zongtao YU
;
Qiong GAO
;
Jun LV
;
Jicai ZHANG
;
Yali YUAN
- Publication Type:Journal Article
- Keywords:
lung carcinoma;
CpG islands methylation;
MS-PCR;
PTEN gene
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the status and diagnostic value of aberrant phosphatase and tensin homology deleted on chromosometen(PTEN) gene promoter in tissues, peripheral plasma and BALF of lung cancer patients. Methods We analyzed the methylation of PTEN gene in tissues, peripheral plasma and BALF by methylation specific-PCR. Results The frequency of methylation of promoter of PTEN gene was 26.67%(12/45) in lung cancer tissues, 15.56%(7/45) in peripheral plasma, 22.22%(10/45) in BALF, no methylation product was found in lung tissues without cancer, normal plasma and BALF controls (P
