Influence of chemical hypoxia induced by cobalt chloride on the proliferation and apoptosis of tumor cells
- VernacularTitle:氯化钴化学模拟低氧对肿瘤细胞增殖及凋亡的影响
- Author:
Jianqun HAN
;
Minghua YU
;
Min DAI
;
Hongwei LI
;
Ruijuan XIU
- Publication Type:Journal Article
- Keywords:
cobalt chloride A549 cell;
HeLa cell;
HIF-1?;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cobalt chloride on tumor cell proliferation and apoptosis in vitro, to explore the reasonable strategy of chemical hypoxia induced by CoCl2. Methods Two tumor cell lines (A549 and HeLa) were respectively exposed to CoCl2(0.05～2 mmol/L) for different time period (4～48 h), cells viability、proliferation and apoptosis were maesured by MTT and FCM methods. HIF-1? and related apoptosis proteins expression were detected by Western blot. Results Cells viability was weakly changed in low concentration(≤200 ?mol/L)of CoCl2 within 24 h. However, higher dose or prolonged challenge of CoCl2 significantly decreased cell survival rate (P
