Effects of riluzole on retinal neovascularization and in vitro cultured human umbilical vein endothelial cells
- VernacularTitle:利鲁唑对体外培养人脐静脉内皮细胞及小鼠视网膜新生血管形成的影响
- Author:
Haixing WU
;
Xuedong ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Studies show that riluzole can greatly inhibit protein kinase C-?Ⅱ in mediating vascular endothelial cell growth factor effects on endothelial cells, so we presume it can be used in inhibiting retinal neovascularization. OBJECTIVE: To explore the inhibitory effects of riluzole on the proliferation of human umbilical vein endothelial cells and retinal neovascularization, and investigate potential mechanism of its effects. DESIGN, TIME AND SETTING: Single-sample observation and randomized controlled animal trial were performed at the Animal Laboratory and Basic Study Laboratory of Chongqing University of Medical Sciences from August to December 2007. MATERIALS: Thirty 3-day-old healthy C57BL/6 mice (60 eyes), irrespective of gender, were selected to model proliferative retinopathy induced by hyperoxia. Raw material of riluzole was product of Venturepharm Laboratories Limited, Beijing (No. 060630, content ≥98.5%). METHODS: Human umbilical vein endothelial cells were cultured, and randomly divided into 3 groups. The mice in hyperoxia model group and riluzole group were modeled, and riluzole group was additionally intraperitoneally injected 10 mg/kg per day riluzole; control group was intraperitoneally injected normal saline. MAIN OUTCOME MEASURES: Effect of riluzole on proliferation of endothelial cell proliferation induced by vascular endothelial growth factor was observed using MTT assay; The inhibitory effects of riluzole on retinal neovascularization were evaluated by counting the endotheliocyte nuclei of new vessels beyond the inner limiting membrane in sections with HE staining. The expression of retinal protein kinase C-?Ⅱand vascular endothelial growth factor were detected with SABC immunohistochemistry. RESULTS: The riluzole of 0.1-10 ?mol/L inhibited proliferation of human umbilical vein endothelial cells in a dose-effect dependent manner. The number of the endotheliocyte nuclei of new vessels beyond the inner limiting membrane was obviously less in eyes of normal control and riluzole groups than in the hyperoxia group (P
