Effect of magnesium sulfute on glutamate-induced cochlear neurotoxicity and intracellular Ca~(2+) in spiral ganglion neuron
- VernacularTitle:镁离子降低耳蜗谷氨酸兴奋毒性及其对耳蜗神经元细胞钙离子内流的影响
- Author:
Aijun LEI
;
Ping WANG
;
Dongmei GAO
;
Bo DU
- Publication Type:Journal Article
- Keywords:
cochlea;
neurons;
glutamic acid,magnesium sulfate
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of magnesium sulfate on glutamate-induced neurotoxicity in cultured cochlear.Methods The cortis of newborn rats were cultivated in vitro. The cultured cochlear neurons were divided into glutamate group treated with 0.1 mmol?L-1 glutamate,magnesium sulfate group treated with different concentrations of magnesium sulfate(0.5,1.0,2.0 and 4.0 mmol?L-1) and combined group treated with glutamate and magnesium sulfate for 24 h. Immunofluorescence staining of Neurofilament 200kDa antibody was used to label spiral ganglion neuron(SGN) and the terminate of dendrites. The morphological changes of SGN were examined by fluorescent microscope and the amount of SGN was determined. In addition,the SGN was loaded with 10 ?mol?L-1 Fluo-3 /AM,and the intracellular Ca2+was measured by laser confocal scanning microscope (LCSM). Results Many SGN were smaller and swelling and vacuoles at the terminate of dendrites following glutamate treatment for 24 h. In combined group treated with glutamate and 0.5,1.0 and 2.0 mmol?L-1 of magnesium sulfate,the amounts of SGN increased compared with glutamate group(P
