Effect of bFGF with different doses on proliferation of human dental pulp cells in vitro
- VernacularTitle:不同浓度bFGF对体外培养人牙髓细胞增殖的影响
- Author:
Xinpeng JIANG
;
Yingli ZHANG
;
Chongtao LIN
;
Hongsheng OUYANG
- Publication Type:Journal Article
- Keywords:
fibroblast growth factor;
dental pulp cells;
cell proliferation
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different concenrtrations of basic fibroblast growth factor (bFGF) on proliferation of human dental pulp cell in vitro,and to find out the most effective concentration of bFGF. Methods Dental pulp cells were isolated from dental pulp tissue explants.The pulp cells were divided into 5 groups randomly,bFGF was added into each group until the ultimately concentrations were 0.1,1.0,10.0 and 100.0 ?g?L-1respectively while the group without bFGF as control group. The effects of bFGF on dental pulp cells were assayed by absorbency A and relative growth rate(RGR) with MTT colorimetric method. Results bFGF at concentrations of 1.0-100.0 ?g?L-1 promoted the cell proliferation (P0.05). Conclusion bFGF has the capability of promoting the proliferation of human dental pulp cells,and the smallest effective concentration is 1.0 ?g?L-1,the most strong cell proliferation takes place at bFGF concentration of 10.0 ?g?L-1.
