Mechanism of programmed cell death of renal proximal tubular cells induced by cisplation
- VernacularTitle:顺铂诱导肾小管上皮细胞程序性死亡的机理
- Author:
Leping FENG
;
Wei QIAO
- Publication Type:Journal Article
- Keywords:
apoptosis;
cisplatin;
Bcl-2 gene;
mitochondria;
endoplasmic reticulum
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the signal pathway of apoptosis of renal proximal tubular cells (RPTC) caused by cisplatin for prevention of toxic effects of cisplatin on kidney. Methods pcDNA3.1/Hygromycin vector and pEGFP-C3 vector including Bcl-2(Bcl-acta,Bcl-cb5 and Bcl-nt) were co-transfected in RPTC.After treated with cisplatin, Bax was activated,cytochrom C release and apoptosis were analyzed with confocal microscope and immunofluoresence technique. Apoptotic cells stained with Hoechst33258 were also counted and statistically analyzed. Results The percent of cytochrom C release (35.74%) in Bcl-cb5 transfected group was higher than those in Bcl-nt group (18.7%) and Bcl-acta group(24.6%)(P
