miR-138 Function and Its Targets on Mouse Mammary Epithelial Cells
- VernacularTitle:miR-138对小鼠乳腺上皮细胞的作用及其调控的靶序列
- Author:
Chunmei WANG
;
Qingzhang LI
;
Ye LI
- Publication Type:Journal Article
- Keywords:
mammary epithelial cell, miR-138, gene silencing
- From:
Progress in Biochemistry and Biophysics
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
miRNA was 20～25 nt endogenous non-coding RNA. miRNAs are encoded small RNAs that hybridize with messenger RNAs, resulting in degradation or translational inhibition of targeted transcripts. In order to investigate the function of miR-138 on mouse mammary epithelial cells, technique for gene silencing-miRNA inhibitor (anti-miRNA) was applied to make miR-138 silence, qRT-PCR was showed valid for inhibitor miR-138. And Western blot, CASY(r)-technology was put in use to study some change of mouse mammary epithelial cells after miR138 inhibitor. It was shown that miR-138 suppresses the exepress of PRL-R(P
