Typing of Resistance Genes of ESBLs from Three Gram-negative Bacilli of Children in Urumqi
- VernacularTitle:乌鲁木齐地区儿童3种革兰阴性杆菌ESBLs耐药基因分型的研究
- Author:
Jun LIU
;
Haozhi YANG
;
Wenli ZHANG
;
Xinghai SHI
- Publication Type:Journal Article
- Keywords:
Children;
Resistant Gram-negative bacilli;
Extended-spectrum ?-lactamase;
Genotyping
- From:
Chinese Journal of Nosocomiology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study antibiotic-resistant phenotypes and genotypes of ESBLs-producing Escherichia coli,Klebsiella pneumoniae and Klebsiella oxytoca isolated from children with pneumonia in Urumqi,to know the distribution and difference of these three Gram-negative bacilli from 2003 to 2007.METHODS Bacterial strains were identified by VITEK32,ESBLs were detected by confirmatory test recommended by CLSI.Microarray technique was used to determine the genotypes of ESBLs.RESULTS Antibiotic-resistant phenotypes showed ESBLs-producing K.oxytoca decreasing from 84.3% to 35.3%,K.pneumoniae stabling in 50-60%,E.coli increasing from 34.4% to 72.1% during the five years;genotypes indicated there were most of ctx-m-9 and tem+ctx-m-9 in E.coli,tem and shv in K.pneumoniae,and the most of tem+ctx-m-3 in K.oxytoca.CONCLUSIONS There is high percentage of ESBLs production from children in Urumqi;resistant phenotypes and genotypes of ESBLs are different in three Gram-negative bacilli;and must further enhance the regional epidemiology surveillance about ESBLs.
