Propargylglycine up-regulated connective tissue growth factor expression in rat pulmonary artery with high pulmonary blood flow
- VernacularTitle:炔丙基甘氨酸增加高肺血流大鼠肺动脉结缔组织生长因子的表达
- Author:
Xiaohui LI
;
Junbao DU
;
Dingfang BU
;
Hongfang JIN
;
Chaoshu TANG
- Publication Type:Journal Article
- Keywords:
H2S;
high pulmonary blood flow;
rat;
endothelin;
connective tissue growth factor
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore possible impact of endogenous hydrogen sulfide(H2S) on connective tissue growth factor(CTGF) expression in rat pulmonary artery with high pulmonary blood flow.Methods Thirty-two male SD rats,weighing 120～140 g,were randomly divided into 4 groups:shunt group,shunt+PPG group,sham group and sham+PPG group.After 4 weeks of experiment,Rat lung tissue H2S content was determined by a modified sulfide electrode method.Plasma ET-1 concentration was detected by radioimmunoactivity,and lung tissue ET-1 mRNA expression of rat was determined by quantitative competitive reverse transcriptional polymerase chain reaction(RT-PCR).Pulmonary artery connective tissue growth factor(CTGF) protein expression of rats was investigated by immunohistochemistry.Results After 4 weeks of experiment,lung tissue H2S content plasma ET-1,lung tissue ET-1 mRNA and CTGF expression increased significantly in rats of shunt group as compared with that of sham group(P
