Effects of TIMP-3 gene transient expression on proliferation,migration and apoptosis of rabbit vascular smooth muscle cells
- VernacularTitle:TIMP-3基因瞬时表达对兔血管平滑肌细胞增殖、迁移及凋亡的影响
- Author:
Jinjie CHEN
;
Xiaodong PU
- Publication Type:Journal Article
- Keywords:
TIMP-3;
vascular smooth muscle cells;
proliferation;
migration;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Observe effects of TIMP-3 gene transient expression on proliferation,migration and apoptosis of VSMCs.Methods Transfect VSMCs separately in three groups as control,empty plasmid and recombinant group mediated by cationic liposome.First,calculate cells,quantify MMP-2 and analyse TIMP-3 mRNA at 24,48 and 72 h;Second,measure cell apoptosis rate and calculate migrated cells as well after 48 h.Results There were no difference between control group and empty plasmid group in cell growth,MMP-2's content,TIMP-3 mRNA expression and apoptosis rate,cells migration rate;but those of recombinant group were greatly different in other two(P
